1、Accelerate New Drug R&D | Precision In Vitro Efficacy Evaluation System Based on Cell Functional Study Platform

2、Service Overview

In vitro drug efficacy research is a critical step in preclinical candidate drug screening. Traditional cell models fail to faithfully mimic the human microenvironment, leading to low clinical translation rates. Leveraging a standardized cell functional study platform, Jennio Bio adopts cutting-edge technologies such as high-content screening and 3D cell culture to build a highly biomimetic in vitro efficacy evaluation system, which significantly shortens drug R&D cycles and lowers clinical failure risks.

3、 Our Services

1. High-Throughput Drug Screening Services
   • Small molecule compound screening: Proliferation inhibition activity of compounds against 60+ human tumor cell lines (lung, gastric, liver cancer, etc.) detected by CCK-8/MTT, with IC50 values and dose-response curve analysis provided.
   • Nucleic acid drug delivery efficiency evaluation: Delivery system efficiency assessed via mRNA transfection (e.g., LUC luminescence), supporting nanocarrier toxicity testing (CCK8/CTG).

2. Cell Mechanism Research Platform

• Cell behavioral analysis: Migration (Transwell), invasion (Matrigel), wound healing, colony formation (plate/soft agar).
• Cell function detection: Apoptosis (Annexin V/PI), cell cycle (PI staining), reactive oxygen species (ROS), mitochondrial membrane potential (JC-1), OCR/ECAR, Mitosox Red/Green.
• Stem cell function evaluation: Osteogenic/adipogenic induced differentiation, tube formation assay.
• Molecular protein detection: qPCR, WB, IHC, IF.

3. In Vitro Immunology Research Platform

• Drug delivery evaluation: T cell detection.
• Immune activation/inhibition study: Multicolor flow cytometry for T cells.
• Elispot assay: T cells, plasma cells, memory B cells / ELISA detection.
• Cell killing function study: CD8+CD108A+.

4. Specialized Model Services

• Primary tumor cell drug sensitivity: 100+ Chinese human primary tumor cell bank, supporting personalized drug response testing.
• 3D organoid rapid drug sensitivity: Liver, colorectal and other organoid models simulating in vivo microenvironment for high-throughput in vitro screening.
• Immune co-culture model: Co-culture of tumor cells with immune cells (e.g., PBMC) to evaluate immunomodulatory drug effects.

4、Technology Platform & Advantages

Technology Platform

• Automated high-throughput platform: Integrated with Molecular Devices microplate readers and automated liquid handling systems, processing 1000+ samples daily with 50% higher efficiency.
• 3D organoid culture platform: Breaks through traditional 2D limitations, improving drug sensitivity prediction accuracy by 40%.
• Live-cell dynamic monitoring: Incucyte real-time imaging system for continuous tracking of cell proliferation, apoptosis and other dynamic responses.

Infrastructure

• Class 10,000 clean cell laboratory: GMP-compliant, equipped with BSL-2 safety facilities.
• Cell resource library: 1000+ cell lines (including 300+ fluorescent tracer lines, drug-resistant lines and gene-edited tool cells).

Differentiated Advantages

• Unique platform supporting combined primary cell and organoid services: One-stop solution from patient samples to efficacy evaluation.
• Cross-platform data integration: Combined with in vivo PDX model data to build a multi-dimensional efficacy evaluation system.

5、Service Process

1. Requirement communication (24h response): customize experimental protocol.
2. Sample preparation: Cell recovery/subculture, with STR identification and mycoplasma detection report provided.
3. Experiment execution: Real-time progress sharing via customer-exclusive platform (e.g., live-cell images, raw data).
4. Data delivery: GLP-aligned standard report (including statistical analysis and methodological validation).

6、Quality Control & Compliance

• Operating specifications: Comply with ISO/IEC 17025, regular calibration of key equipment (annual metrology certification).
• Data traceability: Electronic experimental records, supporting double review and raw data retrieval.
• Cell quality control: All cell lines validated by STR authentication, species verification and sterility testing.

7、Case Studies

Case 1: High-Throughput Screening of Antitumor Drugs

• Client: Top 10 global pharmaceutical company
• Requirement: Screen lead compounds across 15 lung cancer cell lines.
• Outcome: 180 compounds tested within 7 days; 3 candidate molecules with IC50<10μM identified; dose-response curves predict clinical dosage.
• Data support: [Dose-dependent inhibition curve attached]

Case 2: Antibody Immune Effect Evaluation

• Client: European biotechnology company
• Model: PBMC-tumor cell co-culture system.
• Outcome: Accurate assessment of ADCC effect and cytokine release, predicting Phase II clinical efficacy (r=0.89).
• Link: [View more cross-cancer cases]

8、Cooperation Advantages

• Flexible customization: Supports full-cycle protocol adjustment from target validation to IND filing.
• Efficient delivery: Standard project timeline 30% faster than industry average; urgent projects launched within 48h.
• Cost optimization: Pre-deposit of 200,000 RMB enjoys 60% off management fee and 20% off cage fee.
• Global compatibility: Bilingual Chinese-English reports conforming to FDA/EMA formatting requirements.

9、FAQ

Q: Do you support in vitro evaluation of immune checkpoint drugs (e.g., PD-1/PD-L1)?

A: Yes. We have established PD-L1 fluorescent reporter lines and immune co-culture models to detect antibody blocking effects and T cell activation.

Q: What is the culture cycle and success rate of organoids?

A: Standard cycle 4–6 weeks, success rate >85%, with imaging records of the culture process provided.

10、Laboratory Highlights

• Automated high-throughput screening workstation (processing 1000+ samples daily)
• 3D colorectal cancer organoids (brightfield/fluorescent dual-channel imaging)
• BSL-2 cell laboratory (Class 10,000 clean environment)

View More

11、Contact Us

• Technical inquiry: 3691125803@qq.com
• Tel: +86 18802035152
• [Submit inquiry form, 24h response]
• [Download Cell Functional Study Platform Service Manual]