In vitro drug efficacy research

Our Integrated In Vitro Pharmacology Platform

Jennio Biotech’s in vitro drug efficacy research platform is built on four interconnected pillars, each designed to address a specific stage of the drug discovery and evaluation workflow. Together, they provide a seamless pipeline from initial hit identification to in-depth mechanism characterization.

Explore Our In Vitro Efficacy Services

Click on each service below to learn more about our capabilities and how we can support your specific research needs.

Cell Bank — Comprehensive Cell Resources for Drug Discovery

A robust cell model is the foundation of any credible in vitro efficacy study. Jennio Biotech maintains a well-characterized cell bank comprising hundreds of cell lines and primary cells, spanning a wide range of tissue origins, cancer types, and disease-relevant genetic backgrounds.

Our cell bank includes:

• Human cancer cell lines — covering major solid tumors (lung, breast, colorectal, liver, gastric, pancreatic) and hematological malignancies
• Animal cell lines — murine, rat, and other species models for cross-species translational studies
• Asian primary tumor cell lines — patient-derived tumor cells reflecting the genetic diversity of Asian populations, a critical resource for oncology programs targeting regional markets
• Disease-specific and target-overexpressing models — including EGFR, HER2, KRAS, BRAF, PD-1/PD-L1, and other clinically relevant variants

All cell lines undergo rigorous authentication (STR profiling) and routine mycoplasma testing to ensure data integrity and experimental reproducibility. Our team can also source, culture, and bank rare or client-specified cell models on request. Browse Our Cell Bank Catalog →

In Vitro High-Throughput Candidate Drug Screening

Identifying the right compound from thousands of candidates demands speed, precision, and scale. Jennio Biotech’s high-throughput screening (HTS) platform enables rapid, multi-parametric evaluation of compound libraries against carefully curated cell panels tailored to your therapeutic area.

Screening capabilities include:

• Blind Box Cell Panels — discover unexpected sensitivities by screening compounds against diverse, blinded cell collections for serendipitous hit identification
• Cancer Type-Based Panels — focused panels grouped by tumor type (e.g., lung cancer panel, GI cancer panel) for tissue-specific efficacy profiling
• Target-Based Panels — cell panels stratified by molecular marker expression (e.g., EGFR-mutant, HER2-amplified) for precision oncology applications
• Custom Cell Panels — personalized panels designed to match your specific drug targets, disease models, or competitive benchmarking requirements

Our screening infrastructure supports multi-dose, multi-time-point experimental designs with standardized readouts including cell viability (MTT/CCK-8), apoptosis (Annexin V/PI), colony formation, and real-time cell analysis. Data are delivered in comprehensive reports with IC₅₀ values, selectivity indices, and statistical analysis. Explore Screening Options →

Tool Cell Development & Customization

Standard cell models often fall short when studying specific molecular targets, resistance mechanisms, or disease-associated mutations. Jennio Biotech’s tool cell development service creates customized cell lines engineered to meet your exact research specifications.

Our genetic engineering services encompass:

• CRISPR/Cas9 gene-edited cell lines — precise knockout, knock-in, and point mutation models for functional genomics and target validation studies
• Disease-related gene stable strain construction — stable overexpression or knockdown of disease-relevant genes to recapitulate pathological phenotypes in vitro
• Reporter cell lines — cells engineered with luciferase, GFP, or other reporter systems driven by pathway-specific promoters for real-time monitoring of signaling activation
• Primary cell lines from clinical tumor samples — patient-derived primary cultures that preserve the heterogeneity and drug response characteristics of original tumors, ideal for ex vivo drug sensitivity testing

Each engineered cell line undergoes comprehensive validation including genomic confirmation, functional characterization, and stability testing over multiple passages. We provide detailed documentation and quality certificates with every deliverable. Request Custom Cell Line →

Cell Functional Study Platform

Understanding how a compound affects cellular behavior is as important as knowing whether it works. Jennio Biotech’s cell functional study platform provides a comprehensive suite of assays to dissect the phenotypic and mechanistic effects of your drug candidates.

Functional assay categories include:

• Cell proliferation assays — MTT, CCK-8, EdU incorporation, and real-time cell analysis (RTCA) for quantifying growth inhibition and cytostatic effects
• Cell apoptosis and cell cycle analysis — flow cytometry-based Annexin V/PI staining, caspase activity assays, and PI-based cell cycle profiling
• Cell migration and invasion assays — transwell (Boyden chamber), wound healing (scratch), and 3D invasion models for evaluating anti-metastatic potential
• Signal pathway analysis — Western blot, phospho-protein arrays, and reporter gene assays for mapping drug-induced signaling events
• Cell differentiation and stemness studies — for programs targeting cell fate decisions in cancer stem cells, immune cells, or regenerative medicine

Our experienced scientists work closely with you to design assay cascades that align with your program’s stage and decision criteria, ensuring that every experiment generates actionable insights for your drug development team. View Functional Assay Capabilities →

Why Partner with Jennio Biotech for In Vitro Efficacy Studies